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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
Published on: January 20, 2022
Glycan masking in immunogen design: computational and experimental methods
Gustavo Araiza1,2, Josuel Morel1,3, Minh H Tran1,4
1Center for Structural Biology, Vanderbilt University, Nashville, TN, United States.
Abstract:
Epitope-focused vaccine design aims to improve upon existing immunization strategies by eliciting immune responses against specific epitopes targeted by known therapeutic antibodies. One of the techniques in epitope-focused immunogen design is glycan masking, in which sugars are used to hide epitopes on the protein of interest that are associated with low therapeutic potency. Here, we provide a detailed overview of the computational and experimental techniques associated with glycan masking for immunogen design at a biochemical and biophysical level. We will cover well-established and emerging in silico methods for predicting and engineering glycosylation sites. Additionally, we will discuss expression and validation of glycosylated immunogens in vitro. We hope this review will be a useful overview for scientists interested in performing glycan masking in their field of research.
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