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Elucidating the role of SHROOM4 in non-small cell lung cancer: expression patterns, clinical correlations, and
Yuqi Zhang1, Rong Qiang1, Mengyang Ding2
1Center of Medical Genetics, Northwest Women's and Children's Hospital, Xi'an, Shaanxi, China.
Background:
Lung cancer remains the leading cause of cancer-related mortality worldwide, with non-small cell lung cancer (NSCLC) being the most common type. SHROOM4, a protein integral to cytoskeletal organization and cellular signaling, has not been extensively studied in NSCLC.
Methods:
Through bioinformatics analysis of public databases, we investigated the expression of SHROOM4 and its relationship with clinical outcomes and potential mechanism. And we validated the mRNA and protein expression of SHROOM4 in lung squamous cell carcinoma (LUSC) tissues and corresponding normal tissues.
Results:
Our analysis demonstrated a notable downregulation of SHROOM4 mRNA and protein expression, along with its high diagnosis capability in lung cancer, especially pronounced in LUSC, Additionally, higher levels of SHROOM4 were linked to worse clinical outcomes in lung cancer, characterized by reduced survival and more advanced disease stages. Single-cell RNA-seq data and differential analysis show SHROOM4's high expression in stromal cells and its association with angiogenesis and Wnt/Beta-Catenin pathways possibly through ANGPTL7/SFTPC. Meanwhile, SHROOM4 was found to co-express with PTPN13/CACNA1C impacting the tumor microenvironment (TME) and to participate in critical signaling pathways like cell circle and WNT. Moreover, positive correlations were discovered between SHROOM4 expression and immune infiltration scores in NSCLC.
Conclusion:
These results underscore the potential of SHROOM4, an anticancer role, as both a diagnostic and therapeutic target, particularly in LUSC. And SHROOM4 may modulate NSCLC progression by affecting the TME in many ways. Further studies are essential to elucidate SHROOM4's role in lung cancer progression and to validate its clinical utility.
Insights
SHROOM4 protein is downregulated in non-small cell lung cancer (NSCLC), particularly lung squamous cell carcinoma (LUSC). Its expression correlates with poorer outcomes and impacts the tumor microenvironment, suggesting potential as a diagnostic and therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Lung cancer is a leading cause of cancer mortality globally, with non-small cell lung cancer (NSCLC) being the most prevalent subtype.
- The protein SHROOM4, involved in cytoskeletal organization and signaling, has not been thoroughly investigated in the context of NSCLC.
Purpose of the Study:
- To investigate the expression of SHROOM4 in NSCLC using bioinformatics analysis.
- To explore the relationship between SHROOM4 expression, clinical outcomes, and potential mechanisms in lung cancer.
- To validate SHROOM4 mRNA and protein levels in lung squamous cell carcinoma (LUSC) tissues.
Main Methods:
- Bioinformatic analysis of public databases to assess SHROOM4 expression and clinical correlations.
- Validation of SHROOM4 mRNA and protein expression in LUSC tissues versus normal tissues.
- Single-cell RNA sequencing and differential expression analysis to identify cellular localization and associated pathways.
Main Results:
- SHROOM4 expression was significantly downregulated in lung cancer, especially LUSC, demonstrating high diagnostic capability.
- Higher SHROOM4 levels correlated with worse clinical outcomes, including reduced survival and advanced disease stages.
- SHROOM4 expression in stromal cells is linked to angiogenesis and Wnt/Beta-Catenin pathways, co-expresses with PTPN13/CACNA1C, and positively correlates with immune infiltration in NSCLC.
Conclusions:
- SHROOM4 exhibits potential as an anticancer target for diagnosis and therapy, particularly in LUSC.
- SHROOM4 may influence NSCLC progression by modulating the tumor microenvironment.
- Further research is required to fully elucidate SHROOM4's role and clinical utility in lung cancer.
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