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Updated: Jan 24, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Utility of extended HPV genotyping in cervical cancer screening: a prospective population-based cohort study in China
Kexin Yi1,2, Jinyu Zhang1,2, Hong Wang1,2
1Henan Office for Cancer Control and Research, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, Henan, China.
Background:
Cervical cancer poses a substantial global health burden, particularly in China where it contributes substantially to incidence and mortality. Conventional HR-HPV screening tests primarily target HPV16/18, limiting efficacy in regions dominated by other oncogenic genotypes and underscoring the need for extended genotyping to refine risk-stratified approaches.
Methods:
This prospective cohort in Henan Province, China (2017-2020), enrolled 3,299 women aged 21-64 for baseline HR-HPV testing using an extended genotyping assay detecting 14 types and identifying HPV16/18/33/52/58. HR-HPV-positives were followed annually, negatives at year 3. Three-year cumulative CIN2+/CIN3+ risks, sensitivity, specificity, PPV, and NPV were computed independent of cytology.
Results:
Baseline HR-HPV positivity was 18.3%. Over 3 years, 98 CIN2+ and 60 CIN3+ cases were detected. Absolute CIN2+ risks was highest for HPV16 (32.8%), comparable for HPV18/33/58 (25-28%), with relative risks exceeding 96-fold compared to HR-HPV-negatives. Extended genotyping for HPV16/18/33/52/58 improved CIN2+/CIN3+ sensitivity to 55.1% and 60.0%, respectively (vs. 51.0% and 53.3% for HPV16/18), while maintaining specificities >93%.
Conclusions:
In Chinese women, non-16/18 high-risk genotypes exhibit risks comparable to HPV16/18, emphasizing the value of extended genotyping in enhancing risk-based screening sensitivity and efficiency in high-burden contexts.
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