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Forsythiaside a facilitates autophagy to ameliorate chronic nonbacterial prostatitis in rats by blocking the

Xingwei Yu1, Hongao Tan1, Yunqiu Gao1

  • 1Department of Urology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, China.

Frontiers in Molecular Biosciences
|January 23, 2026
PubMed
Abstract

Insights

Forsythiaside A (FTA) effectively treats chronic nonbacterial prostatitis (CNP) in rats by reducing inflammation and promoting autophagy. This natural compound works by inhibiting the protein kinase C alpha (PKCα)/NF-κB pathway, offering a potential new therapy for CNP.

Area of Science:

  • Pharmacology
  • Immunology
  • Cell Biology

Background:

  • Chronic nonbacterial prostatitis (CNP) lacks effective treatments.
  • Forsythiaside A (FTA), a natural compound, exhibits anti-inflammatory properties.
  • Investigating FTA's therapeutic potential for CNP is crucial.

Purpose of the Study:

  • To evaluate the efficacy of forsythiaside A (FTA) in treating chronic nonbacterial prostatitis (CNP) rat models.
  • To elucidate the underlying mechanisms of FTA's action, including its effects on inflammation and autophagy.
  • To assess the impact of FTA on the protein kinase C alpha (PKCα)/NF-κB signaling pathway.

Main Methods:

  • CNP rat models were induced using complete Freund's adjuvant.
  • Rats received FTA (40 and 80 mg/kg/d) for 4 weeks.
  • Prostate damage, inflammatory markers (cytokines, immune cells), autophagic markers, and the PKCα/NF-κB pathway were analyzed.

Main Results:

  • FTA treatment significantly reduced the prostatic index and mitigated prostate damage and inflammatory infiltration.
  • FTA downregulated pro-inflammatory cytokines (IL-1β, IL-2, IL-6, IL-17A, MCP-1, TNF-α) and immune cell markers (CD3, CD45).
  • FTA promoted autophagy (Beclin-1, LC3B II/LC3B I) and inhibited the PKCα/NF-κB pathway (PKCα, p-p65/p65).

Conclusions:

  • Forsythiaside A (FTA) demonstrates significant therapeutic potential for chronic nonbacterial prostatitis (CNP).
  • FTA alleviates inflammation and facilitates autophagy in CNP rat models.
  • The anti-inflammatory effects of FTA are mediated through the inhibition of the PKCα/NF-κB pathway.
Keywords:
NF-κB pathwayautophagychronic nonbacterial prostatitisforsythiaside Aprotein kinase C alpha

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