Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What are Lipids?01:38

What are Lipids?

219.4K
Overview
219.4K
What are Lipids?01:31

What are Lipids?

10.8K
Lipids function as structural components of cellular membranes, in addition to acting as energy reservoirs and signaling molecules. They are thus crucial to all living organisms.  The three biologically important classes of lipids are triglycerides, phospholipids, and steroids.
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and...
10.8K
Lipid Digestion01:06

Lipid Digestion

99.0K
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
99.0K
Structure of Lipids03:38

Structure of Lipids

98.5K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.5K
Blood Studies for Cardiovascular System III: Serum Lipid Profile01:25

Blood Studies for Cardiovascular System III: Serum Lipid Profile

574
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
574
Lipid Catabolism01:25

Lipid Catabolism

893
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
893

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding protein-phospholipid interaction networks in cancer: the role of acyl-chain remodeling.

RSC chemical biology·2026
Same author

Synaptotagmin 1 and Synaptotagmin 7 promote MR1-mediated presentation of <i>Mycobacterium tuberculosis</i> antigens.

eLife·2026
Same author

Deciphering Membrane Protein Complexes in Plasmodium falciparum Gametocytes via Integrative Structural Systems Biology.

Molecular & cellular proteomics : MCP·2026
Same author

A critical signaling role for diacylglycerol in phagocytosis of <i>M. tuberculosis</i>.

bioRxiv : the preprint server for biology·2026
Same author

A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites.

Nature communications·2026
Same author

An ancient monoaminergic signaling system coordinates contractility in a nerveless sponge.

bioRxiv : the preprint server for biology·2026
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jan 24, 2026

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.5K

An Integrated Method for Profiling Lipid-Protein Interactions Using Multifunctional Lipid Probes.

Scotland E Farley, Gaelen Guzman, Berit Blume

    Biorxiv : the Preprint Server for Biology
    |January 23, 2026
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new protocol for mapping cellular lipid-protein interactions. The method identifies specific protein interactors (lipid interactomes) for various lipid species, advancing lipid biology research.

    More Related Videos

    Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
    08:53

    Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

    Published on: January 11, 2017

    9.3K
    Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
    07:33

    Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

    Published on: April 26, 2011

    12.9K

    Related Experiment Videos

    Last Updated: Jan 24, 2026

    Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
    10:02

    Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

    Published on: May 27, 2021

    4.5K
    Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
    08:53

    Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

    Published on: January 11, 2017

    9.3K
    Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
    07:33

    Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

    Published on: April 26, 2011

    12.9K

    Area of Science:

    • Cellular biology
    • Biochemistry
    • Proteomics

    Background:

    • Cellular lipids are crucial in health and disease, but their interactions with proteins (lipid-protein networks) are not well understood.
    • Existing methods for studying lipid-protein interactions are limited, lacking a practical, dedicated protocol.

    Purpose of the Study:

    • To develop and detail a practical protocol for determining lipid interactomes from cells.
    • To enable the identification of protein interactors for specific lipid species.

    Main Methods:

    • Cells are treated with multifunctionalized lipid derivatives and subjected to photochemistry to create lipid-protein conjugates.
    • Click chemistry is employed with a fluorophore for in-gel fluorescence visualization or with azide beads for proteomic analysis.
    • Detailed protocols cover cell treatment, conjugate formation, detection, and sample preparation for proteomics, including controls and troubleshooting.

    Main Results:

    • The protocol successfully enables the mapping of lipid interactomes in diverse biological systems.
    • The workflow allows for the identification of protein partners for specific lipids.
    • The entire process, from cell treatment to proteomic sample preparation, takes approximately 15 hours over four days.

    Conclusions:

    • This protocol provides a practical and dedicated method for studying lipid-protein interactions.
    • It facilitates a significant shift in characterizing the biological roles of lipids by identifying their interactomes.
    • The method is applicable across various biological systems, advancing the field of lipid biology.