Related Experiment Video
Updated: Jan 24, 2026
Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
Published on: February 9, 2020
EBUS-TBNA needle rinse fluid: a superior specimen for the molecular diagnosis of intrathoracic lymph node
Yayan Niu1, Xiaohong Li2, Jin Yu Yan1
1Department of Tuberculosis, The Fifth People's Hospital of Suzhou/The Affiliated Infectious Diseases Hospital, Suzhou Medical College of Soochow University, Suzhou, China.
Objectives:
To evaluate EBUS-TBNA needle rinse fluid versus biopsy tissue for molecular diagnosis of intrathoracic lymph node tuberculosis (TB).
Methods:
Retrospective analysis of 63 patients with intrathoracic lymph node TB undergoing EBUS-TBNA (2018-2024). Rinse fluid and biopsy tissue were tested via TB-DNA (n=32) and Xpert MTB/RIF (n=31); positivity rates compared.
Results:
The study cohort had a median age of 31 years (interquartile range: 25-50.1 years), with 57.1% (36/63) being male. The most frequently sampled lymph node stations were subcarinal (station 7, 82.5%) and right lower mediastinal (station 4R, 66.7%). Clinically, 82.5% (52/63) of patients had concomitant pulmonary TB, while 17.5% (11/63) presented with isolated intrathoracic lymph node TB. For TB-DNA detection, the positivity rate of rinse fluid (71.9%, 23/32) was significantly higher than that of biopsy tissue (46.9%, 15/32; χ²=4.146, P = 0.042). Similarly, the Xpert MTB/RIF assay showed a higher positivity rate in rinse fluid (77.4%, 24/31) compared to tissue (41.9%, 13/31; χ²=8.11, P = 0.004).
Conclusions:
EBUS-TBNA rinse fluid demonstrates higher sensitivity than biopsy tissue for intrathoracic lymph node TB via TB-DNA/Xpert MTB/RIF. Routine rinse fluid testing improves diagnostic yield.
More Related Videos
12:53Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
Published on: May 19, 2018
09:10Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
Published on: December 16, 2013
Related Concept Videos
01:23Detailed Structure and Function of Lymph Nodes
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
01:20Fluid Connective Tissues: Blood and Lymph
Blood
The blood flows through blood vessels— arteries, capillaries, and veins. Blood plasma is primarily made of proteins, solutes, and...
01:29Pulmonary Tuberculosis I
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
01:28Pulmonary Tuberculosis II
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
01:28Pulmonary Tuberculosis V
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
01:22Nursing Diagnosis
The nursing diagnosis focuses on evidence-based...