Related Experiment Video

Updated: Jan 24, 2026

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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Published on: April 29, 2014

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Targeting Senescence: Tianma Granule Inhibits Colorectal Cancer Progression by Modulating miR-29a-5p/P53 Signaling in

Xiaojuan Tang1, Yuan Ren2, Yongmin Li1

  • 1Central Laboratory, Hunan Province Integrated Traditional Chinese and Western Medicine Hospital (Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine), Changsha, China.

Molecular Nutrition & Food Research
|January 23, 2026
PubMed

Insights

Tianma granule (TMG) remodels the tumor microenvironment and inhibits colorectal cancer (CRC) by targeting the miR-29a-5p/P53 axis. This traditional Chinese medicine enhances apoptosis and reduces tumor growth and metastasis.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Chemotherapy induces a senescence-associated tumor microenvironment (S-TME) that promotes colorectal cancer (CRC) progression.
  • Traditional Chinese Medicine (TCM) formulas offer potential therapeutic strategies for cancer treatment.

Purpose of the Study:

  • To elucidate the mechanism by which Tianma granule (TMG) remodels the S-TME and inhibits CRC.
  • To investigate the role of the miR-29a-5p/P53 axis in TMG's anti-CRC effects.

Main Methods:

  • LC-MS/MS identified TMG components; network pharmacology pinpointed P53 as a core target.
  • In vitro assays used doxorubicin-induced senescent cells and CRC lines to assess TMG effects.
  • In vivo studies utilized azoxymethane/dextran sulfate sodium (AOM/DSS)-induced CRC mouse models.

Main Results:

  • TMG suppressed CRC cell proliferation, migration, and invasion, while promoting apoptosis.
  • TMG reduced P53/P21 expression and senescence-associated secretory phenotype (SASP) factors, upregulating miR-29a-5p in senescent cells.
  • TMG treatment in mice decreased tumor burden, improved survival, and modulated the miR-29a-5p/P53 axis in tumor tissues.

Conclusions:

  • TMG remodels the chemotherapy-induced S-TME and suppresses CRC progression.
  • TMG exerts its effects by modulating the miR-29a-5p/P53 axis, enhancing apoptosis in senescent cells.
  • TMG demonstrates therapeutic potential for CRC by counteracting S-TME-mediated tumor growth and metastasis.
Keywords:
Tianma granule miR‐29a‐5p/P53 axiscellular senescence colorectal cancersenescence‐associated tumor microenvironment

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