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Optimizing Decellularized Human Umbilical Vein as a Scaffold for Vascular Tissue Engineering.

Danang Himawan Limanto1,2,3, Thomas Jatiman2, Ni Kadek Sulistyaningsih2

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|January 23, 2026
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Summary

A 0.5% sodium dodecyl sulfate (SDS) solution for 6 hours effectively decellularized human umbilical veins (HUVs), preserving extracellular matrix (ECM) and biocompatibility for vascular tissue engineering.

Keywords:
ECM preservationcardiovascular diseasedecellularization human umbilical veinscaffold biocompatibilitysodium dodecyl sulfatevascular tissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cardiovascular Research

Background:

  • Increasing cardiovascular disease necessitates advanced vascular graft solutions.
  • Decellularized human umbilical vein (HUV) presents a promising biocompatible scaffold.
  • Extracellular matrix (ECM) preservation is key for graft function.

Purpose of the Study:

  • To assess a sodium dodecyl sulfate (SDS)-based decellularization protocol for HUVs.
  • To optimize HUV preparation for subsequent stem cell seeding.
  • To evaluate the efficacy of SDS concentration and duration on HUV decellularization.

Main Methods:

  • Human umbilical veins (HUVs) underwent decellularization using varying SDS concentrations (0.5%, 1%) and durations (6, 12, 24 hours).
  • Scaffolds were analyzed for DNA content, cell viability (MTT assay), collagen/elastin retention, and histological changes.
  • Freeze-drying and gamma-sterilization were employed post-decellularization.

Main Results:

  • The optimal protocol (0.5% SDS, 6 hours) significantly reduced cellularity and DNA content.
  • Cell viability remained high (87.05 ± 17.14%), comparable to controls (84.79 ± 14.3%).
  • Collagen and elastin retention were preserved, indicating intact ECM structure.

Conclusions:

  • The 0.5% SDS, 6-hour protocol is effective for HUV decellularization.
  • This method successfully removes cellular components while maintaining ECM integrity.
  • The prepared HUV scaffolds are suitable for vascular tissue engineering applications.