Related Experiment Video

Updated: Jan 24, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

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Procyanidin B2 Ameliorates Acute Alcoholic Liver Injury by Suppressing the PI3K-Akt/NF-κB Signaling Pathway to

Jianjin Guo1, Zhaohuan Li2, Jing Gao3

  • 1State Key Laboratory of Natural Product Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Journal of Agricultural and Food Chemistry
|January 23, 2026
PubMed

Insights

Procyanidin B2 (PB2) shows promise in treating alcoholic liver injury. This natural compound protects liver cells by targeting key inflammatory and survival pathways, offering a potential new therapy.

Area of Science:

  • Hepatology
  • Pharmacology
  • Biochemistry

Background:

  • Acute alcoholic liver injury (AALI) presents a significant clinical challenge.
  • Its pathogenesis is complex and multifactorial.
  • Effective therapeutic strategies for AALI are limited.

Purpose of the Study:

  • To investigate the hepatoprotective effects of procyanidin B2 (PB2) against AALI.
  • To elucidate the underlying molecular mechanisms of PB2's action.
  • To validate PB2's therapeutic potential through integrated computational and experimental approaches.

Main Methods:

  • Network pharmacology identified key targets and pathways.
  • Molecular docking and dynamics simulations assessed binding affinities.
  • In vivo (mouse model) and in vitro (cellular) experiments validated efficacy.
  • Western blot analysis confirmed pathway modulation.

Main Results:

  • Network analysis highlighted the PI3K-Akt/NF-κB pathway as central to AALI.
  • PB2 exhibited strong binding affinity to core targets like SRC.
  • PB2 treatment attenuated liver damage, oxidative stress, and inflammation in vivo.
  • PB2 alleviated ethanol-induced injury, apoptosis, and inflammation in vitro.
  • PB2 dose-dependently inhibited key signaling pathway phosphorylation.

Conclusions:

  • Procyanidin B2 (PB2) demonstrates significant hepatoprotective effects against acute alcoholic liver injury.
  • PB2 primarily acts by modulating the PI3K-Akt/NF-κB signaling axis.
  • PB2 is a promising natural therapeutic candidate for AALI.
Keywords:
Acute alcoholic liver injuryInflammatory responseNetwork pharmacologyOxidative stressPI3K-Akt/NF-κB PathwayProcyanidin B2

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