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Updated: Jan 24, 2026

Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
Real-Time In Vivo Detection of Nanocarrier Number and Velocity in the Cerebrovasculature Using Hot Band Absorption
Xiaojin Wang1, Mikkel B Liisberg2, Vanessa Rück2
1Department of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
Abstract:
Nanocarriers play a critical role in the transport and targeted delivery of therapeutic agents. However, real-time in vivo observation of individual nanocarriers in the circulatory system is challenging due to the need for high spatiotemporal resolution and sensitivity. Here, DNA-stabilized silver nanoclusters (DNA640), which exhibit anti-Stokes fluorescence upon hot-band absorption (HBA), are loaded into cationic mesoporous silica nanoparticles (CMSN) and coated with liposomes. These constructs (DNA640-CMSN-liposome) exhibit improved resistance to chloride ions (Cl-) compared to bare DNA640, ensuring that they are traceable in the cerebrovasculature. For imaging of the cerebrovasculature, adeno-associated viral vectors (AAV) encoding for albumin-mNeonGreen (Alb-mNG) expression are used to secrete the label into the bloodstream allowing for visualization of the vasculature, exhibiting negligible spectral crosstalk with DNA640. Combining two-photon fluorescence imaging and HBA-based fluorescence correlation spectroscopy enables real-time observation of the flow velocity and particle number of DNA640-CMSN-liposome within individual capillaries, demonstrating potential for pharmacokinetic and hemodynamic studies.
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