Related Experiment Video
Updated: Jan 24, 2026
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Guidelines and Experience Using Imaging Biomarker Explorer IBEX for Radiomics
Published on: January 8, 2018
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The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods
Ziv Yaniv1, Ifeanyichukwu U Anidi2, Leanne Arakkal3
1Bioinformatics and Computational Bioscience Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, United States.
Elife
|January 23, 2026
Summary
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base offers a central platform for researchers using IBEX and immunofluorescence imaging. It promotes open science by sharing data, protocols, and fostering community discussion for faster, more reliable scientific discovery.
Area of Science:
- Biotechnology
- Microscopy
- Open Science
Background:
- Iterative bleaching extends multiplexity (IBEX) is a key method in 2D and 3D immunofluorescence imaging.
- Researchers require centralized resources for adopting and advancing complex imaging techniques.
Purpose of the Study:
- To establish a comprehensive Knowledge-Base for IBEX and related immunofluorescence methods.
- To facilitate open science practices and community collaboration among researchers.
Main Methods:
- Developed a Knowledge-Base modeled on open-source software principles.
- Integrated a development platform (GitHub), static website, and data archiving service.
- Provided validation data for reagents, protocols, videos, datasets, software, and publications.
Main Results:
- Established a central portal for IBEX method users.
- Enabled sharing of positive and negative validation data for reagents, including antibodies.
- Created a forum for community discussion and Q&A.
Conclusions:
- The IBEX Knowledge-Base accelerates scientific discovery by promoting method adoption and evolution.
- Enhanced collaboration and data sharing reduce research waste and increase confidence in results.
- Fostered a global community of researchers advancing immunofluorescence imaging.
Keywords:
cell biologyhumanmousemultiplexed optical imagingopen sciencereagent validationrhesus macaquespatial biologyxenopuszebrafishMore Related Videos
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