Related Experiment Video
Updated: Jan 24, 2026
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
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Guidelines for quantifying ovarian follicles: every follicle counts.
Elyse O K Swindells1, Lauren R Alesi1, Jessica M Stringer1
1Ovarian Biology Laboratory, Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Monash University, Clayton, VIC, Australia.
Reproduction (Cambridge, England)
|January 23, 2026
Summary
Accurate ovarian follicle counting is crucial for reproductive health research. This protocol standardizes methods to improve data reliability and consistency in preclinical studies.
Area of Science:
- Reproductive Biology
- Endocrinology
- Histology
Background:
- Ovarian follicle quantification is essential for assessing ovarian reserve and reproductive health.
- Current manual counting methods lack consistency and are prone to bias, compromising data accuracy.
- Standardized protocols are needed for reliable follicle quantification in research.
Purpose of the Study:
- To provide standardized protocols for accurate and reproducible ovarian follicle quantification.
- To address inconsistencies and biases associated with manual follicle counting methods.
- To support the routine use of follicle number as an outcome in preclinical studies.
Main Methods:
- Outlines key considerations for follicle counting.
- Details two principal methods: direct counting (paraffin-embedded tissue) and stereological analysis (glycol methyl-acrylate sections).
Main Results:
- The protocol aims to ensure consistent, accurate, and objective follicle quantification.
- Standardized approaches enhance data reliability for cross-study comparisons and meta-analyses.
Conclusions:
- Standardized follicle counting protocols are vital for advancing reproductive biology research.
- Adoption of these guidelines will improve the quality and comparability of data on ovarian health and function.
- Consistent follicle quantification supports the assessment of reproductive health in preclinical models.
Keywords:
folliclefollicle countingfolliculogenesismorphologyoocyteovarian reserveovaryprotocolsstereologyMore Related Videos
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