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Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

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When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
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Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
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Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

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Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
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Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

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An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
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Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

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For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
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Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

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Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
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Updated: Jan 25, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
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Infrared Absorption Spectroscopy: A Multifaceted Characterization Tool for Nanomaterials.

Gouranga H Debnath1, Prasun Mukherjee2

  • 1Centre for Nano and Material Sciences, Jain University, Bangalore, Karnataka 562112, India.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 23, 2026
PubMed
Summary

Infrared (IR) spectroscopy reveals more than nanoparticle ligand shells. This technique can also determine ligand coordination, binding mechanisms, and track chemical reactions in nanomaterials.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Spectroscopy

Background:

  • Infrared (IR) absorption spectroscopy is commonly used to identify vibrational modes of nanoparticle (NP) ligand shells.
  • Current applications primarily focus on ligand shell identification.

Purpose of the Study:

  • To highlight the expanded capabilities of IR spectroscopy for nanomaterial characterization.
  • To demonstrate IR spectroscopy's utility beyond ligand shell analysis.

Main Methods:

  • Utilizing IR absorption spectroscopy as a standalone technique.
  • Combining IR spectroscopy with photoluminescence spectroscopy.

Main Results:

  • IR spectroscopy provides data on ligand coordination geometry and binding mechanisms.
  • The technique can track ion exchange and chemical reactions in NPs.
  • IR spectroscopy aids in determining the spatial location of dopants in semiconductor NPs.

Conclusions:

  • IR spectroscopy is a versatile tool for characterizing nanomaterials.
  • Expanded applications of IR spectroscopy can drive new research in nanotechnology and materials science.