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Updated: Jan 25, 2026

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Typhaneoside suppresses osteoclastogenesis and osteoporosis by stabilizing ADORA1

Shaoyan Shi1, Yuan Liu1, Yansheng Huang1

  • 1Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shanxi Province 710000, China.

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
|January 23, 2026
PubMed
Abstract

Insights

Typhaneoside (TYP) reduces bone loss in osteoporosis by inhibiting osteoclast formation. It stabilizes the ADORA1 receptor, offering a potential natural treatment for this bone disease.

Area of Science:

  • Pharmacology
  • Biochemistry
  • Osteoporosis Research

Background:

  • Osteoporosis is a growing concern in postmenopausal women, with current treatments having side effects.
  • Natural compounds like Typhaneoside (TYP) are explored for their anti-inflammatory and antioxidant benefits.
  • The precise mechanism of TYP's effect on osteoclast differentiation is not well understood.

Purpose of the Study:

  • To evaluate the anti-osteoporotic effects of Typhaneoside (TYP).
  • To elucidate the molecular mechanisms of TYP's action on osteoclast differentiation and activity.

Main Methods:

  • Osteoclast differentiation was studied in vitro using mouse bone marrow-derived monocytes (BMMCs).
  • Osteoporosis was induced in vivo using ovariectomy (OVX) in mice.
  • Molecular techniques included TRAP staining, qRT-PCR, Western blot, immunoprecipitation, molecular docking, and mass spectrometry. Bone structure was assessed using Micro-CT and histology.

Main Results:

  • TYP inhibited osteoclast differentiation and bone resorption in vitro without toxicity.
  • In OVX mice, TYP improved bone microarchitecture and reduced osteoclast markers.
  • TYP binds ADORA1, inhibiting NEDD4-1 ubiquitination, stabilizing ADORA1, and suppressing osteoclast gene expression.

Conclusions:

  • Typhaneoside (TYP) effectively reduces osteoclast differentiation and activity.
  • TYP's mechanism involves stabilizing ADORA1 by inhibiting NEDD4-1-mediated ubiquitination.
  • TYP shows promise as a therapeutic agent for treating osteoporosis.
Keywords:
ADORA1NEDD4-1OsteoclastsOsteoporosisTyphaneoside

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