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Updated: Jan 25, 2026

Evaluation of Right Ventricular Function in Experimental Models of Pulmonary Arterial Hypertension
Published on: June 27, 2025
An integrative multi-cohort transcriptomic study reveals cell cycle- and adhesion-related hub genes as diagnostic
Jiajia Zou1, Guojiao Rao2, Xiaomin Hou3
1School of Public Health, Shanxi Medical University, 56 Xinjian South Road, Taiyuan, 030001, Shanxi, China; Environmental Exposures Vascular Disease Institute, Shanxi Medical University, 56 Xinjian South Road, Taiyuan, 030001, Shanxi, China.
Abstract:
Pulmonary arterial hypertension (PAH) is a progressive and fatal vascular disorder for which reliable and non-invasive diagnostic biomarkers remain limited. This study aims to screen and verify the hub genes related to PAH by integrating bioinformatics and experimental verification. We analyzed four GEO datasets (GSE117261, GSE24988, GSE53408, GSE113439) to identify differentially expressed genes and co-expressed modules. Functional enrichment analysis revealed the pathways related to vascular smooth muscle contraction and organ development. Protein-protein interaction network analysis screened out 4 hub genes. These genes showed different expression dysfunctions in the training set, an independent validation set and a Single-cell sequencing dataset (GSE33463, GSE228644), and demonstrated good diagnostic value through ROC curve analysis. Crucially, in the nicotine-induced PAH mouse model, RT-qPCR experiments confirmed the significant upregulation of these 4 genes. Our research results have established CDK1, TPX2, IGF1 and VCAM1 as robust polygenic markers related to PAH, providing potential evidence for clarifying their molecular mechanisms and developing non-invasive diagnostic tools.
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