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Related Concept Videos

Microbial Morphologies01:29

Microbial Morphologies

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Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
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Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...
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Microbial Growth Media01:27

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Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
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Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
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Microbial single-cell omics in situ.

Xihong Lan1, Qiaoxing Liang2, Jinhua He3

  • 1School of Life Sciences, Fudan University, Shanghai 200433, China; State Key Laboratory of Genetics and Development of Complex Phenotypes, Fudan University, Shanghai 200433, China; Greater Bay Area Institute of Precision Medicine (Guangzhou), Fudan University, Guangzhou 511458, China; Central Laboratory of Panyu Central Hospital, The Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou 511400, China.

Cell Genomics
|January 23, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces laser-induced forward transfer (LIFT) for high-quality single-cell microbial genomics and transcriptomics. This method precisely analyzes microbial cells in complex environments, revealing cell-fate and gene expression insights.

Keywords:
LIFTRaman spectrometrycolorectal cancerhost-associated microbiomein situ microscopylaser-induced forward transfermicrobial nicotine metabolismmicrobial single-cell genomesmicrobial single-cell transcriptomescRNAsporulation

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Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Metagenomics is crucial for understanding microbial communities and their functions.
  • Analyzing individual microbial cells within complex environments remains challenging.

Purpose of the Study:

  • To develop and validate a high-throughput method for obtaining high-quality single-cell microbial genomes and transcriptomes.
  • To enable precise analysis of microbial cells in close proximity to each other or host cells.

Main Methods:

  • Utilized laser-induced forward transfer (LIFT) technology for single-cell isolation.
  • Employed fluorescent labeling for visualization and collection of bacterial cells from mouse gut, human saliva, and tumor samples.
  • Generated high-quality single-cell genomes and transcriptomes.

Main Results:

  • Successfully obtained high-quality single-cell genomes and transcriptomes from complex biological samples.
  • Delineated cell-fate commitment during Bacillus sporulation using single-cell transcriptomics.
  • Characterized preliminary gene expression of Bacteroides in a colorectal cancer sample.

Conclusions:

  • LIFT technology provides a scalable and precise method for single-cell microbial analysis.
  • This approach offers novel insights into microbial population dynamics and host-microbe interactions at the single-cell level.