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Temporal microRNA changes and their functional significance during vascular calcification: an integrative

Chiu-Hua Lin1, Hsiang-Yuan Yeh2, Tzu-Ming Jao3

  • 1Graduate Institute of Toxicology, National Taiwan University College of Medicine, Taipei, Taiwan.

International Journal of Biological Macromolecules
|January 23, 2026
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Summary

This study reveals time-dependent microRNA (miRNA) changes in vascular calcification (VC). MiR-202-3p is identified as an early regulator, suggesting it as a potential therapeutic target to inhibit VC progression.

Keywords:
Vascular calcificationmiRNomicmicroRNA

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Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Genomics

Background:

  • MicroRNAs (miRNAs) are critical in vascular calcification (VC) pathogenesis, exhibiting time-sensitive regulation.
  • Limited research exists on temporal miRNA expression patterns and their mechanistic roles in VC progression.

Purpose of the Study:

  • To analyze temporal miRNA and mRNA expression trajectories during VC.
  • To identify key miRNAs and their target genes involved in VC development.
  • To explore the therapeutic potential of targeting specific miRNAs in VC.

Main Methods:

  • Integrated analysis of miRNomic datasets from vascular cells and tissues across multiple time points.
  • Clustering-based trajectory analysis to identify temporal expression patterns of miRNAs and mRNAs.
  • Cross-model validation and functional assays for candidate miRNA-mRNA interactions.
  • In vitro and ex vivo experimental models of VC.

Main Results:

  • Identified six miRNAs with rising trajectories (RT) and 162 target mRNAs with declining trajectories (DT) during VC.
  • Found two miRNAs with declining trajectories (DT) matched with 24 target mRNAs exhibiting rising trajectories (RT).
  • miR-202-3p was confirmed as an early responsive miRNA targeting BTG2, and its inhibition attenuated calcification in experimental models.

Conclusions:

  • Delineated the temporal landscape of miRNA and mRNA expression dynamics in VC.
  • miR-202-3p is a potential early pro-calcific regulator and biomarker for VC.
  • Suppression of miR-202-3p may represent a novel therapeutic strategy for vascular calcification.