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Updated: Jan 25, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Multispectral Laser-Scanning Photoacoustic Microscopy With SRS-Generated Wavelengths for Skin Chromophore
Amir Khansari1, Seyed Mohsen Ranjbaran1, Mohsin Zafar1
1The Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, USA.
Abstract:
We present a single pump-source, multispectral laser-scanning photoacoustic microscopy (MS-LS-PAM) platform that integrates stimulated Raman scattering (SRS)-based wavelength generation for high-resolution, label-free skin imaging. The system uses a compact, fiber-based light source to produce tunable excitation wavelengths from 532 to 571 nm, enabling precise spectral discrimination of key endogenous chromophores, oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), melanin, and collagen, without external contrast agents. Spectral unmixing is performed using non-negative least squares (NNLS), facilitating quantitative analysis of chromophore distributions. In vivo experiments on B6 (pigmented) and SKH1 hairless (melanin deficient) mice demonstrated accurate chromophore separation, spatially resolved oxygen saturation (sO2) mapping, and clear visualization of collagen and melanin architectures.
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