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Multispectral Laser-Scanning Photoacoustic Microscopy With SRS-Generated Wavelengths for Skin Chromophore

Amir Khansari1, Seyed Mohsen Ranjbaran1, Mohsin Zafar1

  • 1The Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, USA.

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|January 23, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel multispectral laser-scanning photoacoustic microscopy (MS-LS-PAM) system for label-free skin imaging. The advanced platform accurately visualizes skin components and oxygen saturation without contrast agents.

Keywords:
cross‐correlationdeep learningmultispectral photoacoustic microscopyphotoacousticsignal decomposition

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Dermatology

Background:

  • Photoacoustic microscopy (PAM) offers label-free imaging capabilities.
  • Distinguishing endogenous chromophores in skin requires precise spectral resolution.
  • Current methods may require exogenous contrast agents or lack quantitative analysis.

Purpose of the Study:

  • To develop and validate a single pump-source, multispectral laser-scanning photoacoustic microscopy (MS-LS-PAM) platform.
  • To enable high-resolution, label-free imaging of endogenous skin chromophores.
  • To perform quantitative analysis of chromophore distributions and oxygen saturation.

Main Methods:

  • Integration of stimulated Raman scattering (SRS)-based wavelength generation for tunable excitation (532–571 nm).
  • Utilizing a compact, fiber-based light source for spectral discrimination of oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), melanin, and collagen.
  • Employing non-negative least squares (NNLS) for spectral unmixing and quantitative analysis.

Main Results:

  • Accurate spectral separation of endogenous chromophores in vivo.
  • Spatially resolved quantitative mapping of blood oxygen saturation (sO2).
  • Clear visualization of collagen and melanin architectures in B6 and SKH1 mice models.

Conclusions:

  • The developed MS-LS-PAM platform provides high-resolution, label-free skin imaging.
  • The system enables precise quantification of chromophore distributions and physiological parameters like sO2.
  • This technology holds potential for advanced dermatological diagnostics and research.