Recombinase-aided amplification technology: A comprehensive review of innovations and multi-technology integration...

Recombinase-Aided Amplification (RAA), as an emerging isothermal nucleic acid amplification technique, demonstrates significant potential in infectious disease diagnostics due to its advantages of rapidity, high efficiency, and reduced reliance on complex instrumentation. Amid frequent global outbreaks of infectious diseases, the demand for rapid and accurate diagnostic technologies has intensified. RAA, leveraging its unique amplification mechanism and application flexibility, has garnered increasing research interest. Compared to other amplification techniques, RAA exhibits shorter reaction times and higher sensitivity, enabling the detection of diverse pathogens including viruses, bacteria, fungi, and parasites. Furthermore, the integration of RAA with techniques such as CRISPR and microfluidics provides innovative strategies for point-of-care testing. However, current RAA implementation faces industrial bottlenecks such as low primer design tolerance, nonspecific amplification due to sample matrix interference, and lack of standardized frameworks. This review aims to summarize the fundamental principles of RAA technology, its current applications in infectious disease diagnosis, synergistic innovations with complementary methods, and future development trajectories, thereby offering theoretical insights for the optimization and advancement of rapid diagnostic technologies.