Related Experiment Video

Updated: Jan 25, 2026

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

7.6K

RecO: a potential target for overcoming fluoroquinolone resistance in Pasteurella multocida

Xin-Yu Li1, Xuan-Yu Li2, Jie He3

  • 1College of Animal Science and Technology, Department of Veterinary Medicine, Jilin Agricultural University, Xincheng Street #2888, Changchun 130118, China.

Journal of Applied Microbiology
|January 24, 2026
PubMed
Abstract

Insights

Inhibiting the recO gene in Pasteurella multocida (Pm) reduces fluoroquinolone resistance and delays its development. This finding suggests the recO gene as a potential target for improving fluoroquinolone efficacy against Pm.

Area of Science:

  • Bacteriology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • Pasteurella multocida (Pm) is a primary cause of bovine respiratory disease.
  • Antibiotic resistance and tolerance mutations in Pm pose challenges for disease control.
  • Fluoroquinolones are commonly used antibiotics against Pm.

Purpose of the Study:

  • To investigate the role of the recO gene in mediating Pm resistance and tolerance to fluoroquinolones.
  • To identify potential targets for enhancing fluoroquinolone efficacy against Pm.

Main Methods:

  • Utilized highly pathogenic Pm strains (sensitive and enrofloxacin-induced resistant).
  • Employed RNA-sequencing to screen SOS response-related genes.
  • Functionally verified the recO gene's role using MIC and MBC assays.

Main Results:

  • recO deletion significantly reduced bacterial tolerance to enrofloxacin (10-100-fold).
  • Deletion of recO decreased the MBC value by 2-fold.
  • recO deletion prolonged the time required for fluoroquinolone resistance development.

Conclusions:

  • Inhibiting recO gene expression in Pm reduces fluoroquinolone resistance.
  • Targeting the recO gene can delay the emergence of fluoroquinolone resistance.
  • The recO gene represents a potential therapeutic target to enhance fluoroquinolone activity against Pm.
Keywords:
PmrecOSOS responseantibiotic tolerancefluoroquinolones resistance

More Related Videos

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.7K
Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

11.1K

Related Concept Videos

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems01:22

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems

Body:Bioavailability is a critical pharmacological concept that measures the extent and rate at which an active drug ingredient or therapeutic moiety enters the systemic circulation, remaining unchanged. It's a pivotal factor in determining a drug's efficacy and safety.The Biopharmaceutics Classification System (BCS) plays an essential role in drug development by categorizing drugs into four classes based on their solubility and permeability. This classification aids in understanding drug...
199
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.4K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
5.7K
Potential Energy00:52

Potential Energy

The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.4K
Resistance and Conductance01:25

Resistance and Conductance

A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
499
Equivalent Resistance01:16

Equivalent Resistance

In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
948