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Related Experiment Video

Updated: Jan 26, 2026

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Published on: January 13, 2023

VPS34-IN1 potentiates STING-dependent activation in human CAL-1 cells.

Paulo Antas¹, Mariana D Machado¹, Fátima Leite-Pinheiro^1,2

¹Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193, Aveiro, Portugal.

Cellular & Molecular Biology Letters

|January 24, 2026

Summary

This summary is machine-generated.

VPS34-IN1 minimally impacts cancer cells but inhibits Toll-like receptor 7 signaling. This compound activates STING, enhancing type I interferon production for potential antitumoral immunity.

Keywords:

BPDCN CL307 Chemotherapy Immunotherapy PtdIns 3-kinase STING Type I interferon

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Last Updated: Jan 26, 2026

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Published on: January 13, 2023

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Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Published on: October 20, 2022

Area of Science:

Immunology
Cell Biology
Pharmacology

Background:

Vacuolar protein sorting 34 (VPS34) is a kinase involved in endosome dynamics.
VPS34 inhibition is explored as a cancer therapy.
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematologic malignancy.

Purpose of the Study:

To investigate the effects of VPS34-IN1 on BPDCN cells.
To assess VPS34-IN1's impact on Toll-like receptor (TLR) and stimulator of interferon genes (STING) signaling pathways.

Main Methods:

Treatment of BPDCN CAL-1 cells with VPS34-IN1.
Analysis of TLR7 signaling inhibition.
Assessment of STING pathway activation and type I interferon (IFN) production.

Main Results:

VPS34-IN1 showed minimal cytotoxic effects on BPDCN CAL-1 cells.
VPS34-IN1 inhibited TLR7 signaling but activated STING.
Enhanced cellular response to 2'3'-cyclic guanosine monophosphate-adenosine monophosphate (2'3'-cGAMP) and increased type I IFN expression were observed.

Conclusions:

VPS34-IN1 has limited direct toxicity against BPDCN cells.
VPS34-IN1 synergizes with STING activation to promote type I IFN expression.
VPS34-IN1 shows potential for enhancing antitumoral immunity via type I IFN induction.