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Cell surface microvilli and cell agglutinability.

J H Temmink, J G Collard

    Cell Biology International Reports
    |March 1, 1977
    PubMed
    Summary
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    Surface microvilli on detached mouse fibroblasts do not determine their agglutination with Concanavalin A (ConA). Cell surface morphology differences are not reflected in ConA agglutinability, suggesting microvilli number is not the primary factor.

    Area of Science:

    • Cell biology
    • Biophysics

    Background:

    • Transformed mouse fibroblast cell lines exhibit variations in surface morphology when detached.
    • Surface characteristics of detached cells, such as villous or smooth appearance, are not consistently linked to their in situ morphology.

    Purpose of the Study:

    • To investigate the relationship between cell surface morphology, specifically microvilli, and agglutinability with Concanavalin A (ConA) in mouse fibroblasts.
    • To determine if changes in cell surface morphology correlate with altered ConA agglutination responses.

    Main Methods:

    • Comparative analysis of surface morphology (villous vs. smooth) of detached transformed mouse fibroblast cell lines.
    • Assessment of agglutinability with Concanavalin A (ConA) in normal and transformed Swiss mouse fibroblasts.
    • Induction of changes in agglutinability using specific treatments and observation of corresponding morphological changes.

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    Main Results:

    • Differences in surface morphology of detached fibroblasts (villous vs. smooth) did not correlate with their agglutinability by ConA.
    • Treatments altering ConA agglutinability in fibroblasts did not induce equivalent changes in cell surface morphology.
    • No clear link was established between the number of microvilli and the agglutination response to ConA.

    Conclusions:

    • The agglutinability of mouse fibroblasts by Concanavalin A is unlikely to be determined by the number of microvilli on the cell surface.
    • Cell surface morphology, particularly microvilli, is not the sole or primary determinant of ConA-mediated cell adhesion in these fibroblast models.