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Published on: July 27, 2018
Genome-based reclassification of the genus Thermoanaerobacter: taxonomic emendations and new combinations
Noureddine Bouras1, Guendouz Dif2, Saïd Belghit3
1Laboratoire de Valorisation et Conservation des Ecosystèmes Arides (LVCEA), Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaia, B.P. 455, Ghardaïa, Algeria; Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure Cheikh Mohamed El Bachir El Ibrahimi, BP 92, Kouba, Algiers, Algeria.
Objective:
The genus Thermoanaerobacter is comprised of strictly anaerobic, thermophilic, rod-shaped bacteria predominantly isolated from geothermal environments. Due to phylogenetic and taxonomic overlaps among certain species, this study aims to clarify the taxonomic status of key Thermoanaerobacter members through genome-based analyses.
Methods:
We conducted a comprehensive taxonomic re-evaluation using multiple genomic metrics, including digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI via FastANI), average amino acid identity (AAI), and percentage of conserved proteins (POCP). These analyses were supported by 16S rRNA gene sequencing and genome-based phylogenomic reconstruction.
Results:
Genome-based metrics and phylogenomic analyses revealed several taxonomic inconsistencies within the genus. As a result, Thermoanaerobacter thermohydrosulfuricus is redefined to include Thermoanaerobacter ethanolicus subsp. thermohydrosulfuricus, Thermoanaerobacter ethanolicus subsp. siderophilus, Thermoanaerobacter ethanolicus subsp. ethanolicus and Thermoanaerobacter ethanolicus subsp. indiensis under Thermoanaerobacter thermohydrosulfuricus subsp. thermohydrosulfuricus subsp. nov., while Thermoanaerobacter wiegelii is assigned to Thermoanaerobacter thermohydrosulfuricus subsp. wiegelii subsp. nov., comb. nov. Thermoanaerobacter pseudethanolicus, Thermoanaerobacter brockii subsp. finnii, and Thermoanaerobacter brockii subsp. lactiethylicus DSM 9801T were reclassified as Thermoanaerobacter brockii. Additionally, Thermoanaerobacter mathranii, Thermoanaerobacter italicus, and Thermoanaerobacter pentosaceus are reclassified as Thermoanaerobacter thermocopriae.
Conclusions:
This study supports a taxonomic rearrangement within Thermoanaerobacter, refining species boundaries based on robust genomic evidence. The proposed changes enhance the taxonomic resolution and phylogenetic consistency of thermophilic anaerobic bacteria within this genus.
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