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MA-T Activates Human TRPV4 in the Non-pigmented Ciliary Epithelial Cells
Qianwen Luo1, Manami Toriyama1, Miho Hashimoto1,2
1Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University (Currently known as The University of Osaka), 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
None:
Matching transformation system (MA-T), an on-demand aqueous chlorine dioxide production solution is utilized in various applications, including antibacterial and cancer therapies. Herein, we focused on the ability of MA-T in oxidizing human transient receptor potential cation channel subfamily V member 4 (hTRPV4), which functions as a redox sensor, using Ca2+ imaging and patch-clamp experiments. We found that 10 ppm MA-T activated hTRPV4 in HEK293T cells. Moreover, MA-T induced increase in cytosolic calcium concentration through hTRPV4 activation in human non-pigmented ciliary epithelial cells. Our study findings suggest MA-T as a potential agent for TRPV4-related diseases.
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