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Published on: December 1, 2017
Cell-free systems for automation and robotics
Juveriya Israr1, Shabroz Alam2, Ajay Kumar3
1Institute of Biosciences and Technology, Shri Ramswaroop Memorial University, Barabanki, Uttar Pradesh, India; Department of Biotechnology Era University, Lucknow, Uttar Pradesh, India.
None:
The amalgamation of cell-free systems with automation and robotics is transforming the domains of biotechnology and bioengineering. This chapter examines the swiftly advancing domain of robotic platforms utilizing cell-free protein synthesis (CFPS) and other programmable cell-free biological mechanisms. Cell-free systems have substantial benefits compared to conventional cell-based approaches, such as less contamination risk, improved control over reaction variables, and expedited response times. These attributes render them exceptionally appropriate for high-throughput screening, expedited prototyping of synthetic biology circuits, on-demand biomanufacturing, and point-of-care diagnostics. This chapter offers a detailed examination of the design, functionalities, and constraints of automated cell-free systems, emphasizing technologies such as microfluidic devices, liquid handling robots, and integrated analytical platforms. Principal difficulties addressed encompass standardization, the creation of more resilient and economical cell-free extracts, and the incorporation of artificial intelligence and machine learning for enhanced experimental design and process optimization. The integration of cell-free system programmability with the accuracy and scalability of automation and robotics is set to expedite discovery, facilitate the development of innovative biomaterials, and broaden access to advanced biotechnological tools and applications.
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