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Updated: Jan 28, 2026

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Making deep immunophenotyping accessible: the successful application of a guided 23-parameter mouse immunophenotyping

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|January 26, 2026
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Summary

This study presents a cost-effective, reproducible 23-color mouse immunophenotyping panel for high-parameter spectral flow cytometry. The resource streamlines workflow and lowers entry barriers for researchers using shared resource facilities.

Keywords:
antibodiesflow cytometryimmune phenotypingmousepanel design and optimization

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Area of Science:

  • Immunology
  • Biotechnology
  • Analytical Chemistry

Background:

  • High-parameter spectral flow cytometry enables detailed cellular analysis.
  • Shared resource facilities (SRFs) can enhance researcher support through specialized panels.
  • Developing standardized, accessible protocols is crucial for broad adoption.

Purpose of the Study:

  • To develop and present a versatile 23-color mouse immunophenotyping panel.
  • To provide a reproducible and cost-effective method for high-parameter spectral flow cytometry.
  • To reduce barriers for researchers accessing advanced flow cytometry techniques.

Main Methods:

  • Design and validation of a 23-color immunophenotyping panel for mouse samples.
  • Optimization of reagents, staining protocols, and instrument setup.
  • Development of acquisition and analysis templates, including manual gating and dimensionality reduction (tSNE, uMAP).

Main Results:

  • The panel is broadly applicable across multiple tissue types.
  • The protocol is modifiable, reproducible, and cost-effective.
  • Implementation in a shared resource facility requires minimal staff preparation for maximum researcher benefit.

Conclusions:

  • Shared resource facilities can offer significant value by providing accessible, high-parameter flow cytometry resources.
  • This panel serves as a complete resource for institutions to adopt and adapt.
  • The approach lowers entry barriers, increases reproducibility, and reduces costs for researchers in spectral flow cytometry.