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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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Complement and Coagulation Cascades Pathway was Inactivated in HIV-Associated Colorectal Cancer: Results from a

Shixian Lian1, Lei Li1,2, Yuexiang Yang1

  • 1Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.

Journal of Cancer
|January 26, 2026
PubMed
Summary

Human Immunodeficiency Virus (HIV)-1 infection alters colorectal cancer (CRC) proteomic profiles. HIV-CRC shows distinct ribosome upregulation and complement/coagulation cascade downregulation, with C8B and SERPINA1 as potential biomarkers.

Keywords:
HIVcolorectal cancercomplement and coagulation cascadesproteomicsribosome

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Area of Science:

  • Proteomics
  • Cancer Biology
  • Virology

Background:

  • Colorectal cancer (CRC) is a major global health concern.
  • Human Immunodeficiency Virus (HIV)-1 infection is linked to poorer CRC outcomes.
  • Understanding HIV's impact on CRC mechanisms is crucial.

Purpose of the Study:

  • To investigate the proteomic differences in colorectal cancer (CRC) associated with HIV-1 infection.
  • To identify specific protein expression patterns and affected pathways in HIV-positive CRC patients.
  • To validate potential protein biomarkers for HIV-associated CRC.

Main Methods:

  • Tandem Mass Tag (TMT) proteomics was performed on tumor and adjacent normal tissues from HIV-positive and HIV-negative CRC patients.
  • Differential protein expression analysis was conducted, focusing on HIV-specific changes.
  • KEGG pathway enrichment analysis was used to identify significantly altered biological pathways.
  • Key differentially expressed proteins (DEPs) were validated using western blot and immunohistochemistry.

Main Results:

  • A total of 592 HIV-specific differentially expressed proteins (DEPs) were identified in HIV-associated colorectal cancer (CRC).
  • Upregulated ribosome proteins (40) and downregulated complement and coagulation cascades (CCC pathway; 24 proteins) were the most significantly altered pathways.
  • Downregulation of key CCC pathway proteins, C8B and SERPINA1, was confirmed, suggesting their potential as biomarkers.

Conclusions:

  • HIV-associated CRC exhibits unique proteomic alterations compared to HIV-negative CRC.
  • Dysregulation of the ribosome and CCC pathways are key features of HIV-CRC.
  • C8B and SERPINA1 emerge as promising biomarkers for HIV-associated colorectal cancer.