Related Experiment Video
Updated: Jan 28, 2026
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
Published on: March 31, 2022
Construction and Expression Analysis of the bin 3xOllas Tool Line
Mengyuan Yi1, Hannah Sonnenberg1, Vimala Anthonydhason2
1Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Västra Götaland, Sweden.
None:
Precise detection of endogenous protein expression is essential for understanding gene function in vivo . We generated a Drosophila knock-in line inserting a 3×Ollas tag at the C-terminus of the endogenous biniou ( bin ) locus, enabling specific visualization of the Bin transcription factor. The bin 3xOllas allele faithfully recapitulates native expression in the visceral mesoderm and does not disrupt Bin function. Integrating single-cell RNA-sequencing data, we further identified Bin expression in adult reproductive tissues, including ovarian escort cells and testis hub and cyst cells. This allele provides a robust tool for studying Bin function and dynamics.
More Related Videos
Related Concept Videos
01:13Overview of Microsoft Excel as a Data Analysis Tool
01:24Bode Plots Construction
01:42What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
01:15Construction of Root Locus
For positive gain values, the root locus exists on the real axis to the left of an odd number of finite open-loop poles or zeros. The root locus starts at the open-loop poles and traces the paths of the closed-loop poles as the gain...
01:15Construction of Frequency Distribution
First, make a table with two columns—one with the title of the data that needs to be organized, and the other column for frequency. [Draw a third column for tally marks if needed]. Then, take a look at the items given in the data set and decide if an ungrouped frequency distribution table or a grouped frequency distribution table would be more suitable. If there are large sets of different values, then it is...
02:48Expressing Solution Concentration
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.