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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
Published on: December 20, 2013
Novel Imidazopyridine-Oxadiazole β‑Tubulin Inhibitors Suppress Breast Cancer Migration and Induce Caspase-3-Mediated
1Department of Medical Biology, Faculty of Medicine, Van Yuzuncu Yil University, Van 65080, Türkiye.
Abstract:
A series of novel imidazo-[1,2-a]-pyridine-oxadiazole (iMPZ1-15) hybrid compounds was designed as potential β-tubulin polymerization inhibitors, inspired by the cis-conformation and biaryl scaffold of combretastatin A-4. The effects of iMPZ beta-tubulin inhibitors on proliferation in MDA-MB-231, SH-SY5Y, and DLD-1 cancer cells, as well as their influence on beta-tubulin inhibition, colonization, cell migration, cell cycle progression, and apoptosis in MDA-MB-231 cells, were investigated. iMPZ-8 identified as the most efficacious treatment candidate, with an IC50 value of 7.5 μM in MDA MB-231 cells. iMPZ-8 had a comparable effectiveness to NOC, which served as a positive control for beta tubulin inhibition. IMPZ-8 reduced cellular migration and colonization. It also accumulated throughout the G2/M phase of the cell cycle, through the BAX-Caspase-3 intrinsic apoptotic signaling pathway.
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