Related Experiment Video

Updated: Jan 28, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.4K

Rapid colistin susceptibility determination by a novel fluorogenic method in Klebsiella pneumoniae strains

Meliha Negin1,2, Meltem Ayas3,4, Isin Akyar4

  • 1Department of Medical Biotechnology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul, Türkiye.

Frontiers in Microbiology
|January 26, 2026
PubMed
Abstract

Insights

A novel fluorometric method rapidly determines colistin resistance in Klebsiella pneumoniae within 2 hours. This aids timely treatment decisions for critical infections, unlike standard 24-48 hour tests.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Klebsiella pneumoniae causes severe infections, particularly in intensive care units.
  • Colistin is a last-resort antibiotic for multidrug-resistant K. pneumoniae.
  • Rising colistin resistance necessitates rapid susceptibility testing.

Purpose of the Study:

  • To evaluate a novel fluorometric method for determining colistin minimal inhibitory concentration (MIC).
  • To compare the accuracy and speed of the fluorometric method against standard susceptibility testing.

Main Methods:

  • Determined MIC values for 42 K. pneumoniae strains (24 resistant, 18 susceptible).
  • Compared results from a standard broth microdilution method with a new fluorometric assay.
  • Assessed method accuracy based on MIC value agreement and differences.

Main Results:

  • The fluorometric method yielded identical MIC values for 22 strains.
  • A 1-dilution difference was observed in 14 strains, and a 2-dilution difference in six strains.
  • The novel method provides results within 2 hours, significantly faster than standard methods.

Conclusions:

  • The fluorometric method accurately detects colistin resistance in K. pneumoniae.
  • This rapid test is a valuable tool for guiding colistin treatment in critically ill patients.
  • Faster susceptibility results can improve patient outcomes and antibiotic stewardship.
Keywords:
Klebsiella pneumoniaecolistin resistancefluorometric assayminimum inhibitory concentrationrapid antimicrobial susceptibility testing

More Related Videos

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers08:11

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

2.2K
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing11:25

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

36.5K

Related Concept Videos

Pneumonia I: Introduction01:30

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
852
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
2.8K
Magnetic Susceptibility and Permeability01:31

Magnetic Susceptibility and Permeability

In linear magnetic materials, like paramagnets and diamagnets, magnetization is proportional to the magnetic field intensity. The constant of proportionality, a dimensionless number, is called magnetic susceptibility. The value of the susceptibility depends on the type of material.
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
2.4K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
646
Pneumonia IV: Management01:28

Pneumonia IV: Management

The treatment of pneumonia varies based on its severity and the causative pathogen. Here is a structured approach to managing pneumonia, integrating pharmaceutical and supportive care strategies.
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
803
Susceptibility, Permittivity and Dielectric Constant01:26

Susceptibility, Permittivity and Dielectric Constant

When placed in an external electric field, a dielectric material gets polarized. The charge density in the dielectric material is given by the sum of the bound and free charge densities, while the total charge density can also be written in terms of the total electric field. The bound charge density can be measured in terms of polarization, leading to the relationship between electric displacement and polarization.
2.8K