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Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Biosynthesis of Nucleic Acids01:28

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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic Acids and Nucleotides01:20

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
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Single-Molecule Nucleic Acid Detection with a Reconfigurable Rotating DNA Origami Nanodevice.

Emily Tsang1, Line M Lund1, Victoria Birkedal1

  • 1Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Aarhus C 8000, Denmark.

ACS Nano
|January 26, 2026
PubMed
Summary
This summary is machine-generated.

Researchers created a reusable DNA nanodevice for continuous nucleic acid sensing. This DNA origami device achieves low nanomolar detection limits and offers insights into molecular dynamics.

Keywords:
DNA nanotechnologyDNA origamifluorescencenanodevicesingle molecule spectroscopystrand displacement

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Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Molecular Biology

Background:

  • DNA nanodevices offer programmable platforms for nanoscale engineering.
  • Macroscopic machines inspire the design of dynamic nanodevices with specialized functions.

Purpose of the Study:

  • To develop a DNA origami-based rotating nanodevice for continuous nucleic acid sensing.
  • To demonstrate the reversibility and regeneration capabilities of the nanodevice for multiple detection rounds.
  • To design single-mode and dual-mode nanodevices for Förster Resonance Energy Transfer (FRET) and multiplexed measurements.

Main Methods:

  • Utilized DNA origami for precise nanoscale construction.
  • Employed toehold-mediated strand displacement for reversible target detection.
  • Applied ensemble and single-molecule techniques to analyze nanodevice dynamics and conformational changes.
  • Designed single-mode (FRET) and dual-mode (FRET/quenched) systems for varied detection strategies.

Main Results:

  • Achieved a detection limit in the low nanomolar range for nucleic acid sensing.
  • Demonstrated successful regeneration of the nanodevice for multiple detection cycles.
  • Obtained high-resolution insights into dynamic conformational changes at the single-molecule level.
  • Successfully implemented multiplexed measurements using the dual-mode nanodevice.

Conclusions:

  • The developed DNA origami nanodevice enables continuous and reversible nucleic acid sensing.
  • Single-molecule analysis provides valuable insights into the dynamic behavior of nanodevices.
  • The programmable nature of DNA origami allows for versatile sensor design, including multiplexed detection.
  • This work offers a foundation for optimizing DNA nanodevice design for enhanced sensing applications.