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Updated: Jan 28, 2026

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
Published on: March 24, 2013
KIF5B-driven unfolded protein response reprograms breast cancer immunosuppressive microenvironment for single-cell
Yue Liu1, Shuyu Li1, Zhiyong Luo1
1Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Background:
Breast cancer (BRCA) remains a leading cause of cancer-related mortality among women worldwide. The kinesin family member 5B (KIF5B) has been implicated in various cancers, yet its comprehensive role in BRCA prognosis, tumor microenvironment (TME), and therapeutic response remains poorly understood.
Methods:
We integrated single-cell RNA sequencing (scRNA-seq) data and bulk RNA-seq data from multiple datasets. Using hdWGCNA, we identified gene modules related to the unfolded protein response (UPR). Consensus clustering defined UPR-related molecular subtypes, and differential expression analysis revealed key prognostic genes. KIF5B was further evaluated for its prognostic significance, mutational landscape, immune infiltration associations, and potential as a therapeutic target.
Results:
Malignant cell-specific KIF5B upregulation drives poor overall survival in pan-cancer cohorts, validating its hazardous molecular function. Functional enrichment analysis linked KIF5B to critical pathways, including immune response, JAK-STAT signaling, and epithelial-mesenchymal transition. Immune infiltration analysis revealed that high KIF5B expression was associated with reduced immune and ESTIMATE scores, but higher tumor purity. Drug sensitivity prediction identified several compounds with potential efficacy in high KIF5B-expressing patients, including Sapitinib and LCL161.
Conclusion:
Our multimodal analysis establishes KIF5B as a prognostic biomarker and potential therapeutic target in BRCA, with implications for understanding immune evasion and guiding precision treatment strategies.
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