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Published on: August 29, 2017
Anatoxin biosynthesis gene abundance correlates with anatoxin concentration in freshwater benthic samples
Joshua R Evans1, Daniel G Beach2, Jake Stillwell1
1Department of Biology, University of New Brunswick, 10 Bailey Drive, Fredericton, E3B 5A3, New Brunswick, Canada.
None:
The abundance of the anaC gene, which is involved in anatoxin biosynthesis, correlated with anatoxin concentration (rs = 0.80, p < 0.0001) in Atlantic Canadian benthic freshwater samples. Average total anatoxins (100 μg g-1 by high resolution mass spectrometry) and anaC (4.7 x 108 copies g-1 by droplet digital PCR) were highest in August, while highest average toxin quota (8.9 pg anaC-1) was observed in July. Pairing approaches provided insight into the seasonality of anatoxin biosynthesis.
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