Related Experiment Video
Updated: Jan 28, 2026

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica
Published on: September 20, 2024
BoMYC2 and BoAREB1 Interact With BoMYB2 to Respond to ABA Signaling and Drought Stress in Cabbage
Zhen Shen1, Shuhua Huang2, Minghui Guo1
1College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Abstract:
The MYB family has been extensively studied in model organisms, but research on these transcription factors in vegetable crops such as cabbage (Brassica oleracea L. var. capitata L.) remains incomplete. We cloned BoMYB2 and determined its role in cabbage drought response. We found that overexpression of BoMYB2, which is induced by ABA, significantly enhanced cabbage tolerance to drought, whereas BoMYB2-silenced line displayed the opposite phenotype. Two proteins that interact with BoMYB2, BoMYC2 and BoAREB1, were verified by yeast two-hybrid, luciferase complementation, pull-down assays. Overexpression of these genes in Arabidopsis thaliana significantly improved drought tolerance in A. thaliana, while their transient silencing in cabbage seedlings reduced drought tolerance. Both proteins are also induced by ABA, and they cooperate with BoMYB2 to enhance plant antioxidant capacity under drought, modulate downstream gene expression, and increase plant survival during water deficit. In summary, our results indicate that BoMYB2-BoMYC2 and BoMYB2-BoAREB1 complexes play important roles in ABA-signal-mediated regulation of drought responses in cabbage.
More Related Videos
Related Concept Videos
Responses to Drought and Flooding
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Responses to Heat and Cold Stress
Responses to Salt Stress
Yeast Signaling
What is Cell Signaling?

