Related Experiment Video
Updated: Jan 28, 2026
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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
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Human P53 Expression in Yeast: Investigating Its Apoptotic Effects.
Sihem Ben Abid1, Wafa Mihoubi1, Ines Yacoubi2
1Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, Sidi Mansour Street, km 6.5, Sfax, 3018, PB 1177, Tunisia.
Mini Reviews in Medicinal Chemistry
|January 27, 2026
Summary
The tumor suppressor p53 negatively impacts yeast cell growth, inducing apoptosis. This yeast model facilitates cancer research, including screening and serological diagnosis.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- The tumor suppressor p53, discovered in 1979, is crucial in various organisms.
- Yeast serves as an effective model for studying p53's roles and functions.
- Previous research focused on specific aspects of p53, particularly its apoptotic function.
Purpose of the Study:
- To review team studies on p53 expression in yeast.
- To investigate the apoptotic function of p53 in yeast models.
- To establish a yeast system for p53 activity verification and cancer research.
Main Methods:
- Utilizing Saccharomyces cerevisiae and Pichia pastoris strains for p53 expression studies.
- Developing a yeast model for p53 activity verification using cDNA without UTR.
- Implementing a screening mechanism for antioxidant/anti-apoptotic molecular activity.
Main Results:
- Confirmed a negative effect of high-dose p53 on yeast cell growth, leading to apoptosis.
- Demonstrated that the NLS signal directs p53 to both mitochondria and nucleus.
- Successfully tested co-expression with thioredoxin 2 (TRX2) and impact of Nigella sativa extracts.
Conclusions:
- The developed yeast model confirms p53's negative effect on cell growth and induction of apoptosis.
- This system enables assessment of cancer mutants and revertants, evolving into a screening mechanism.
- High-yield yeast p53 production supports applications in serological cancer diagnosis.
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