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Updated: Jan 28, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
The Influence of Polysaccharides on the Textural Properties and Water Retention Capacity of Animal-Plant Dual-Protein
Wenhao Gao1,2, Zhiming Wang2, Zhihao Zhao2
1College of Life Sciences, Yangtze University, Jinzhou 430070, China.
Abstract:
To develop nutrient-rich whole-food gels for individuals with dysphagia, this study constructed a pork-whole soy milk composite gel (PSG) using a hybrid animal-plant protein approach. The effects of xanthan gum, konjac glucomannan, and guar gum at different concentrations (0.5%, 1.0%, and 1.5%) on the gel properties, protein conformation, and microstructure of different PSGs were systematically investigated. The results indicated that polysaccharides interfered with protein cross-linking and disrupted the gel network, leading to reduced gel hardness. Due to their abundant hydrophilic groups, the polysaccharides significantly enhanced the water-holding capacity (p < 0.05), achieving a synergistic outcome of structural softening and functional reinforcement. A comprehensive evaluation identified the PSG with 1.0% xanthan gum as the optimal formulation, which exhibited a 43.2% increase in water-holding capacity and a hardness only 23.5% of the control, complying with both International Dysphagia Diet Standardisation Initiative (IDDSI) Level 5 and Japanese Dysphagia Diet Level III standards. This study elucidates the mechanism by which polysaccharides modulate whole-food protein gels and provides a practical strategy for developing dysphagia-friendly foods that preserve nutritional quality and are suitable for industrial production.
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