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Updated: Jan 28, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Highly Sensitive Biosensor for the Detection of Cardiac Troponin I in Serum via Surface Plasmon Resonance on
Alice Marinangeli1, Pinar Cakir Hatir2,3, Mustafa Baris Yagci4
1Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
Abstract:
In this work, we report the development of a highly sensitive optical sensor for the detection of cardiac troponin I (cTnI), a key biomarker for early-stage myocardial infarction diagnosis. The sensor combines castor oil-derived biomimetic receptors, called GreenNanoMIPs and prepared via the molecular imprinting technology using as a template an epitope of cTnI (i.e., the NR10 peptide), with a portable multimode plastic optical fiber surface plasmon resonance (POF-SPR) transducer. For sensing, gold SPR chips were functionalized with GreenNanoMIPs as proven by refractive index changes and confirmed by means of XPS. Binding experiments demonstrated the cTnI_nanoMIP-SPR sensor's ability to detect both the NR10 peptide epitope and the full-length cTnI protein within minutes (t = 10 min), with high sensitivity and selectivity in buffer and serum matrices. The cTnI_nanoMIP-SPR showed an LOD of 3.53 × 10-15 M, with a linearity range of 1 pM-100 pM, outperforming previously reported sensor platforms and making it a promising tool for early-stage myocardial infarction detection.
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