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Updated: Jan 28, 2026
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Magnetic Europium Ion-Based Fluorescence Sensing Probes for the Detection of Tetracyclines in Complex Samples
Miftakhul Jannatin1, Yu-Chie Chen1,2
1Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan.
None:
Eu3+ is a fluorescent and paramagnetic ion whose emission intensity increases when chelated with enhancers such as tetracycline (TC). In this study, Eu3+ was conjugated with citric acid (CA) to form magnetic fluorescent probes capable of capturing trace TC from solutions. The probes were rapidly prepared (~2.25 min) and trapped TC within ~2.5 min under microwave heating. The method enabled sensitive detection of TC, oxytetracycline, and chlortetracycline with detection limits of ~3-7 nM by fluorescence spectroscopy. It was successfully applied to real food samples, including fresh chicken broth and commercial broth cubes, achieving high accuracy (93.7% and 96.6%). This dual-functional probe offers a rapid and sensitive approach for detecting TC residues in complex food matrices, demonstrating strong potential for food-safety monitoring.
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