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Engineered PVA Hydrogel as a Universal Platform for Developing Stable and Sensitive Microbial BOD-Biosensors.

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|January 27, 2026
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Summary

Highly sensitive biosensors were developed using modified polyvinyl alcohol (PVA) hydrogels for rapid biochemical oxygen demand analysis. This innovation offers a stable and effective platform for environmental water quality monitoring.

Keywords:
BODBlastobotrys adeninivoransbiosensorhydrogelspolyvinyl alcoholtwo-mediator system

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Area of Science:

  • Materials Science
  • Biotechnology
  • Environmental Science

Background:

  • Polyvinyl alcohol (PVA) hydrogels are versatile materials with potential for biosensor applications.
  • Enhancing PVA's physicochemical properties is crucial for developing stable and sensitive bioanalytical systems.
  • Existing methods for biochemical oxygen demand (BOD) analysis can be time-consuming and lack sensitivity.

Purpose of the Study:

  • To investigate modified PVA hydrogels as a platform for highly sensitive biosensors.
  • To develop a rapid and reliable method for biochemical oxygen demand (BOD) analysis in water.
  • To improve the stability and performance of biosensors for environmental monitoring.

Main Methods:

  • PVA hydrogels were modified using radical polymerization under UV irradiation and Ce4+ ion treatment.
  • A dual-mediator system with ferrocene (FC) and neutral red (NR) was employed.
  • Yeast *Blastobotrys adeninivorans* was immobilized within the modified PVA matrix.

Main Results:

  • The modified PVA hydrogels exhibited enhanced mechanical strength, stability, and biocompatibility.
  • The FC+NR-*B. adeninivorans*-PVA-Ce4+ biosensor showed high sensitivity (linear range 0.1-3.81 mgO2/dm3) and selectivity.
  • The biosensor demonstrated operational stability for up to 37 days and strong correlation with standard BOD5 tests in wastewater.

Conclusions:

  • Radical modification of PVA hydrogels is an effective strategy for creating sensitive and stable biosensors.
  • The developed biosensor offers a promising platform for rapid and accurate water quality monitoring.
  • This approach advances environmental monitoring technology with a simple and efficient method.