Transcriptomic Insights into Metabolic Reprogramming and Exopolysaccharide Synthesis in Porphyridium purpureum Under Gradual Nitrogen Deprivation
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Nitrogen deprivation in red microalgae redirects carbon to produce valuable sulfated exopolysaccharides (EPS). This study reveals key carbohydrate-activated enzymes (CAZymes) involved in EPS biosynthesis under stress.
Area Of Science
- Marine biotechnology
- Microalgal physiology
- Biochemical engineering
Background
- Red microalgae, such as *Porphyridium* species, are recognized for producing bioactive sulfated exopolysaccharides (EPS) with significant industrial and biomedical applications.
- While abiotic stresses like nitrogen deprivation influence EPS production, the precise molecular mechanisms and the role of carbohydrate-activated enzymes (CAZymes) in EPS biosynthesis remain largely unelucidated.
- Understanding these mechanisms is crucial for optimizing EPS production in microalgae.
Purpose Of The Study
- To investigate the physiological, biochemical, and transcriptomic responses of *Porphyridium purpureum* to progressive nitrogen deprivation.
- To elucidate the metabolic regulation and identify key carbohydrate-activated enzymes (CAZymes) involved in exopolysaccharide (EPS) biosynthesis under nitrogen-limited conditions.
- To establish a link between nitrogen availability, carbon metabolism, and EPS production.
Main Methods
- Integrated physiological, biochemical, and transcriptomic analyses (RNA-Seq) of *P. purpureum* under progressive nitrate consumption.
- Quantitative real-time PCR (RT-qPCR) was employed for transcriptomic data validation.
- Analysis focused on metabolic shifts, EPS release, and the expression of CAZyme families.
Main Results
- *P. purpureum* exhibited a phase-dependent metabolic adaptation to nitrogen stress, with EPS release correlating to reduced nitrate uptake.
- Transcriptomic analysis revealed a global metabolic downregulation coupled with the upregulation of stress-responsive pathways, carbohydrate catabolism, and nucleotide-sugar synthesis.
- Specific upregulation of CAZyme families GT4, GT8, and GT77 was observed, indicating their potential role in EPS biosynthesis.
Conclusions
- Nitrogen and carbon metabolic pathways are coordinately regulated to govern polysaccharide biosynthesis in *P. purpureum* under nitrogen stress.
- The study provides insights into the molecular mechanisms driving EPS production in response to nutrient limitation.
- Findings open avenues for future research into enzyme compartmentalization and flux distribution in nitrogen-stressed microalgae.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Gradually varying flow (GVF) in open channels describes situations where water depth changes slowly along the channel due to factors like non-uniform bed slope, channel shape variations, or obstructions. This flow type occurs when the depth adjusts gradually to balance gravitational forces, shear forces, and energy requirements, resulting in a low rate of depth change.Characteristics of Gradually Varying FlowGVF is commonly observed in natural streams, rivers, and canals, where flow depth...
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
Overview
Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes.
Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
Insufficient sleep refers to not getting the recommended amount of sleep for optimal functioning, even if it's just slightly less than needed. Sleep insufficiency may occur due to lifestyle choices, such as staying up late for social events or work, resulting in routinely getting less sleep than required. For example, consistently sleeping 6 hours when the body needs 7-9 hours can lead to cumulative effects on health and well-being.
Sleep deprivation is a more severe form of sleep loss...
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...