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Development of Organogel-AFPT System for Encapsulation and Cryopreservation of Yarrowia lipolytica
Ziying Zhang1, Ruiqi Cui2, Haoyu Yang1
1Department of Bioengineering, School of Synthetic Biology and Biomanufacturing, State Key Laboratory of Synthetic Biology, and Frontier Science Center for Synthetic Biology, Tianjin University, Tianjin 300350, China.
Abstract:
Encapsulation of microorganisms is a promising strategy in biomedical and industrial applications to enhance stability, reusability, and functionality. However, the cryopreservation of microorganism-encapsulated materials still represents a formidable challenge because of the mechanical and osmotic damage caused by inevitable ice formation at subzero temperatures. In this study, we developed a hydrophobic antifreeze material (organogel-AFPT) specifically for oleaginous microorganisms, following the principle of reducing the overall water content of the system and inhibiting ice nucleation and growth. By the cryoprotection of organogel-AFPT, 94% survival rate of Yarrowia lipolytica (Y. lipolytica) can be achieved after cryopreservation at -196 °C. It is also found that the organogel-AFPT can serve as a convenient platform for information encryption and decryption. The findings in this work provide a new strategy for the development of antifreeze living materials.
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