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Quantitative 31P NMR Analysis of Lignins and Tannins

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Quantitative Chiral Analysis by Molecular Rotational Spectroscopy Using Noncovalent Derivatization.

Luca Evangelisti¹, Brooks H Pate²

¹Department of Chemistry Giacomo Ciamician, University of Bologna, Ravenna, Italy;

Annual Review of Physical Chemistry

|January 27, 2026

Summary

This summary is machine-generated.

A new chiral tag rotational spectroscopy technique enables routine chiral analysis of small organic molecules. This method determines enantiomeric excess without requiring analyte reference samples.

Keywords:

absolute configuration chirality enantiomeric excess high-resolution molecular spectroscopy molecular clusters molecular rotational spectroscopy

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Area of Science:

Physical Chemistry
Analytical Chemistry

Background:

Chiral analysis of small organic molecules is crucial in various scientific fields.
Existing methods often require reference samples or extensive sample preparation.

Purpose of the Study:

To introduce a novel technique for routine chiral analysis.
To enable determination of absolute configuration and enantiomeric excess without analyte reference standards.

Main Methods:

Chiral tag rotational spectroscopy utilizes chiral derivatization to form diastereomeric complexes.
Complex formation occurs during molecular beam expansion for Fourier transform microwave spectroscopy.
Computational chemistry methods guide the analysis of tag complex geometries.

Main Results:

The technique successfully converts enantiomers into spectroscopically distinct diastereomers.
Absolute configuration of analytes can be assigned through spectral analysis.
Enantiomeric excess is determined by analyzing spectral intensity changes.

Conclusions:

Chiral tag rotational spectroscopy offers a powerful new tool for chiral analysis.
The method's ability to forgo analyte reference samples significantly simplifies the process.
This technique advances the routine chiral analysis of small organic molecules.