Related Experiment Video
Updated: Jan 29, 2026

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
Published on: May 23, 2014
White Light Excited Two-Dimensional Fluorescence Excitation Spectroscopy: Picosecond Time-Resolved Excitation
Tatsuya Yoshida1, Kiyoshi Miyata1, Ken Onda1
1Department of Chemistry, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan.
Abstract:
Two-dimensional electronic spectroscopy (2DES) has been widely employed to probe the dynamics of electronic excitations. However, conventional 2DES often encounters challenges in spectral assignment due to the intrinsic limitations of transient absorption detection. To overcome these issues, fluorescence-detected 2DES has been anticipated, and broadband excitation sources can enhance its performance. In this study, we have developed a two-dimensional fluorescence excitation (2DFLEX) spectroscopy by combining white-light excitation with Kerr-gate detection. This approach exploits the white light generated from a high-repetition-rate Yb-amplifier laser, providing both broad spectral coverage and excellent stability. Importantly, 2DFLEX exhibits intrinsic selectivity for stimulated emission, enabling the isolation of excited-state dynamics. This technique represents a significant advancement in fluorescence-detected 2DES and opens new opportunities for elucidating complex electronic processes. 2DFLEX is a spectroscopic technique that integrates excitation-spectrum measurements with time-resolved photoluminescence and has the potential to become a novel standard analytical method for photofunctional materials.
More Related Videos
Related Concept Videos
Excitation-Contraction Coupling in Skeletal Muscles
When an action...
Atomic Spectroscopy: Absorption, Emission, and Fluorescence
Emission Spectra
Atomic Emission Spectroscopy: Lab
Molecular Spectroscopy: Absorption and Emission
Atomic Emission Spectroscopy: Overview

