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Updated: Jan 29, 2026

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
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Effective delivery of genome editor to cervical cancer targeting Mcl1 for cancer therapy

Yue Wan1, Yiming He2, Xun Chen1

  • 1Department of Gynecology, Sir Run Run Hospital, Nanjing Medical University, Long Mian Avenue 109 Jiangning, Nanjing, Jiangsu, China.

Cancer Gene Therapy
|January 27, 2026
PubMed

Insights

This study engineered artificial extracellular vesicles (EVs) to deliver CRISPR/Cas9 gene editing tools, effectively inhibiting cervical cancer growth and progression in preclinical models.

Area of Science:

  • Biotechnology
  • Gene Editing
  • Cancer Therapy

Background:

  • CRISPR/Cas9 technology offers precision therapy potential but faces delivery and off-target editing challenges.
  • Effective delivery systems are crucial for expanding CRISPR/Cas9 applications in cancer treatment.

Purpose of the Study:

  • To engineer an endogenous artificial extracellular vesicle (EV) system for efficient delivery of Cas9 ribonucleoprotein (RNP).
  • To evaluate the therapeutic efficacy of EV-delivered Cas9 RNP in inhibiting cervical cancer progression.

Main Methods:

  • Engineered artificial EVs for endogenous Cas9 RNP sorting and delivery via Lamp2b.
  • Assessed the impact of EV-delivered Cas9 RNP on cervical cancer cell growth and apoptosis in vitro.
  • Evaluated the antitumor effects of artificial endogenic EVs (Cas9-Mcl1) in animal models of cervical cancer.

Main Results:

  • Demonstrated successful sorting and delivery of Cas9 RNP by engineered artificial EVs.
  • Observed significant inhibition of cervical cancer cell growth through induced apoptosis.
  • Achieved remarkable antitumor effects in vivo by suppressing Mcl1 expression using artificial endogenic EVs.

Conclusions:

  • Artificial EVs provide an effective delivery strategy for Cas9 RNP, overcoming current limitations.
  • This EV-based delivery system shows promise for inhibiting cancer progression and warrants further investigation as a novel therapeutic approach.

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