Cathelicidin-like Peptide for Resistant Acinetobacter baumannii Control.
Elizabete de Souza Cândido1,2, Danieli Fernanda Buccini2, Elizangela de Barros Miranda2
1Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, DF, Brazil.
Antibiotics (Basel, Switzerland)
|January 28, 2026
Summary
Snake venom peptides BotrAMP14 and CrotAMP14 show potent antibacterial and antibiofilm activity against drug-resistant skin pathogens. These antimicrobial peptides offer a promising alternative for treating challenging wound infections.
Area of Science:
- Antimicrobial resistance research
- Peptide therapeutics
- Wound infection management
Background:
- Antimicrobial resistance (AMR) poses a global health threat, especially in wound infections.
- Multidrug-resistant pathogens like Acinetobacter baumannii form biofilms, complicating treatment.
- Antimicrobial peptides (AMPs) offer a novel therapeutic approach with reduced resistance potential.
Purpose of the Study:
- To evaluate snake venom-derived cathelicidin-like peptides against multidrug-resistant bacteria.
- To assess the antibacterial, antibiofilm, and in vivo efficacy of selected peptides.
- To investigate the safety profile, including hemolytic and cytotoxic effects, of these peptides.
Main Methods:
- Determined Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), and Minimal Biofilm Inhibitory Concentration (MBIC).
- Conducted time-kill kinetics, hemolytic activity, and cytotoxicity assays.
- Utilized a murine skin wound infection model to assess in vivo efficacy and safety.
Main Results:
- Peptides showed potent activity against planktonic and biofilm forms of resistant bacteria (MIC/MBC: 0.78-25 µM; MBIC: 1.56-12.5 µM).
- BotrAMP14 and CrotAMP14 demonstrated rapid bactericidal action and significant in vivo efficacy in a murine wound model.
- Peptides exhibited no hemolytic activity and low cytotoxicity, indicating a favorable safety profile.
Conclusions:
- BotrAMP14 and CrotAMP14 are effective topical antimicrobial agents against multidrug-resistant skin infections.
- These peptides represent a promising alternative to conventional antibiotics for challenging wound infections.
- The findings address the urgent need for novel therapies against antibiotic-resistant pathogens.
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