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Related Concept Videos

Factors Affecting Protein-Drug Binding: Protein-Related Factors01:20

Factors Affecting Protein-Drug Binding: Protein-Related Factors

551
Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
551
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
15.0K
Drug Distribution: Plasma Protein Binding01:29

Drug Distribution: Plasma Protein Binding

8.8K
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
8.8K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

648
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
648
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.7K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.7K
Protein-Drug Binding: Mechanism and Kinetics01:16

Protein-Drug Binding: Mechanism and Kinetics

1.8K
Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
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Updated: Jan 29, 2026

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
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The Zinc Finger Ran-Binding Protein 3 (ZRANB3): An Advanced Perspective.

Paride Pelucchi1, Ettore Mosca1, Nika Tomsič2

  • 1Istituto di Tecnologie Biomediche (ITB), Consiglio Nazionale delle Ricerche (CNR), Via Fratelli Cervi 93, 20054 Milano, Italy.

International Journal of Molecular Sciences
|January 28, 2026
PubMed
Summary
This summary is machine-generated.

Human ZRANB3 protein is vital for DNA damage tolerance and genome stability, acting as a tumor suppressor. Its altered expression in cancer correlates with poor survival, highlighting its prognostic potential.

Keywords:
ATP-ase translocaseDNA repaircancerfork remodelertumour suppressor

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Human zinc finger Ran-binding protein 3 (ZRANB3) is essential for DNA damage tolerance (DDT).
  • ZRANB3 functions in replication fork reversal (RFR) and the p53/Polι pathway, implicating it as a tumor suppressor.
  • Deregulation of ZRANB3 in tumors is linked to adverse clinical outcomes.

Purpose of the Study:

  • To comprehensively review the gene and protein-level literature on ZRANB3.
  • To examine ZRANB3 regulation in cancer development.
  • To discuss evidence for ZRANB3's role as a tumor suppressor and prognostic biomarker.

Main Methods:

  • Literature review integrating gene and protein data.
  • Analysis of ZRANB3's role in DNA damage tolerance and replication fork dynamics.
  • Examination of ZRANB3 alterations (mutations, copy number) in cancer.

Main Results:

  • ZRANB3 maintains genome stability by preventing DNA damage and restoring fork progression.
  • ZRANB3 expression is frequently deregulated in tumors, correlating with poor prognosis.
  • The correlation between ZRANB3 mRNA and p53 expression is context-dependent, particularly in p53-mutated cancers.

Conclusions:

  • ZRANB3 is a critical tumor suppressor involved in DNA repair pathways.
  • Altered ZRANB3 is a significant prognostic biomarker in various cancers.
  • Further research into ZRANB3's functions in DNA synthesis and metabolism is warranted.