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Related Experiment Video

Updated: Jan 29, 2026

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

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Published on: May 2, 2018

Tuber Inoculation Drives Rhizosphere Microbiome Assembly and Metabolic Reprogramming in Corylus.

Jing Wang^1,2, Nian-Kai Zeng¹, Xueyan Zhang¹

¹Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 570228, China.

International Journal of Molecular Sciences

|January 28, 2026

Summary

This summary is machine-generated.

Tuber inoculation transforms the soil microbiome, shifting it towards mutualism and enhancing plant defense. This integrated multi-omics study reveals how mycorrhizal fungi act as ecosystem engineers, promoting plant-microbe symbiosis.

Keywords:

community assembly functional succession metabolic reprogramming multi-omics integration rhizosphere microbiome

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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

Published on: June 3, 2020

Area of Science:

Plant-microbe interactions
Mycorrhizal symbiosis
Metagenomics and metabolomics

Background:

Understanding the systemic effects of mycorrhizal fungi on plant-microbe interactions is crucial.
Integrated multi-omics approaches offer a powerful tool to investigate these complex relationships.

Purpose of the Study:

To elucidate the potential of integrated multi-omics approaches in studying mycorrhizal fungi.
To investigate how Tuber inoculation reshapes the rhizosphere microbial community and host metabolism in Corylus plants.

Main Methods:

Pot experiment comparing inoculated (Tuber) and non-inoculated Corylus plants.
High-throughput sequencing (16S/ITS) for microbial community profiling.
Functional prediction (PICRUSt2/FUNGuild) and metabolomics (UPLC-MS/MS) for functional and metabolic analysis.

Main Results:

Tuber inoculation significantly restructured the fungal community, promoting symbiotic guilds and suppressing pathogens.
Bacterial community function shifted towards symbiotic support and environmental adaptation.
Root metabolism was reprogrammed, with altered strigolactone and gibberellin levels, indicating a 'symbiosis-priority' strategy.

Conclusions:

Integrated multi-omics successfully captured multi-level changes in Tuber-Corylus symbiosis.
Tuber acts as a core regulator, collaborating with the host to create a mutualistic micro-ecosystem.
Mycorrhizal fungi function as 'ecosystem engineers,' transforming the rhizosphere and providing a framework for green agriculture.