Related Experiment Video
Updated: Jan 29, 2026
12:21
Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
Published on: December 13, 2014
13.1K
Distinct Serum MicroRNA Signatures and mRNA Decay Pathway Dysregulation in NSAID-Exacerbated Chronic Urticaria
Young-Min Ye1, Jin Young Noh2,3,4, Seung Ho Kim3,4
1Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
International Journal of Molecular Sciences
|January 28, 2026
Summary
Nonsteroidal anti-inflammatory drug (NSAID) hypersensitivity in chronic urticaria (CU) is linked to specific microRNA (miRNA) changes. Identifying these miRNA signatures may help in diagnosing and treating NSAID-exacerbated CU.
Area of Science:
- Immunology
- Genetics
- Dermatology
Background:
- Nonsteroidal anti-inflammatory drugs (NSAIDs) can worsen chronic urticaria (CU) in a significant patient subset.
- This NSAID-exacerbated CU (NECU) subtype involves distinct inflammatory and leukotriene-driven pathways.
- MicroRNAs (miRNAs) are key regulators of immune and inflammatory processes.
Purpose of the Study:
- To identify differentially expressed miRNAs (DEMs) in patients with NSAID-exacerbated CU (NECU) compared to NSAID-tolerant CU (NTCU).
- To elucidate the molecular networks and biological pathways associated with these DEMs in NECU.
- To explore the potential of miRNAs as biomarkers for disease endotyping and therapeutic stratification in CU.
Main Methods:
- Serum miRNA profiling was performed on 14 NECU patients and 16 NTCU patients using an Affymetrix GeneChip miRNA 4.0 Array.
- Differentially expressed miRNAs (DEMs) were identified based on fold change (>1.5) and statistical significance (p < 0.05).
- Validated miRNA targets were retrieved from the multiMiR database for network construction and Gene Ontology enrichment analysis.
Main Results:
- NECU patients showed a higher incidence of angioedema and required more systemic corticosteroid treatment.
- Eight DEMs were identified, with notable upregulation of miR-5001-5p, miR-4270, miR-6869-5p and downregulation of miR-6511b-5p, miR-2277-5p, miR-378h in NECU.
- Network analysis highlighted key clusters (e.g., AGO2-BTG2-LMNB2) involved in mRNA decay and inflammatory signaling, suggesting a role for impaired post-transcriptional regulation.
Conclusions:
- Distinct miRNA expression profiles differentiate NECU from NTCU patients.
- A potential miR-5001-5p/miR-6511b-5p-mRNA decay axis may link impaired post-transcriptional regulation to leukotriene-driven inflammation in CU.
- Identified miRNAs represent promising biomarkers for endotyping NECU and guiding targeted therapies.
Related Concept Videos
02:27Nonsense-mediated mRNA Decay
11.8K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.8K
02:27Nonsense-mediated mRNA Decay
3.4K
3.4K
01:22MicroRNAs
24.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
02:48Radioactive Decay and Radiometric Dating
37.1K
Radioactivity is a spontaneous disintegration of an unstable nuclide and is a random process, as all the nuclei in the sample do not decay simultaneously. The number of disintegrations per unit time is called the activity (A), which is directly proportional to the number of nuclei in the sample. The decay constant (λ) is an average probability of decay per nucleus in unit time.
37.1K
02:51mRNA Stability and Gene Expression
6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.6K
02:31Nuclear Export of mRNA
8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K