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Bacterial Cellulose Production by a Novel Levilactobacillus brevis Isolate Using Response Surface-Optimised
Panyot Mongkolchat1,2, François Malherbe1, Enzo Palombo1
1Department of Chemistry and Biotechnology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Foods (Basel, Switzerland)
|January 28, 2026
Summary
This study demonstrates sustainable bacterial cellulose (BC) production using agro-industrial waste. A novel lactic acid bacterium, Levilactobacillus brevis DSS.01, produced BC with optimized waste medium, significantly reducing costs.
Area of Science:
- Biotechnology
- Microbial Production
- Sustainable Materials
Background:
- High costs of culture media limit bacterial cellulose (BC) production.
- Agro-industrial wastes are underutilized carbon and nitrogen sources.
- Valorizing waste streams can reduce production expenses and environmental impact.
Purpose of the Study:
- To sustainably bioconvert agro-industrial waste into bacterial cellulose (BC).
- To optimize BC production using a novel lactic acid bacterium, Levilactobacillus brevis DSS.01.
- To evaluate the economic feasibility and material properties of BC produced from waste.
Main Methods:
- Isolation and screening of BC-producing bacteria from nata de coco wastewater.
- Identification of optimal carbon (pear pomace) and nitrogen (rice bran) sources from Australian agro-industrial wastes.
- Response surface methodology (Box-Behnken Design) for medium optimization.
- Characterization of BC using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Diffraction (XRD).
Main Results:
- Levilactobacillus brevis DSS.01, Acetobacter tropicalis KBC, and Komagataeibacter xylinus TISTR 086 were evaluated.
- Optimal medium composition for L. brevis DSS.01 yielded 1.56 ± 0.15 g/L BC.
- Optimized waste medium replaced 85% of standard Hestrin-Schramm medium, significantly cutting costs.
- BC produced from waste showed uniform fiber diameter and minimal structural deviation compared to standard medium production.
Conclusions:
- Agro-industrial waste can be effectively valorized for cost-effective and sustainable bacterial cellulose production.
- Levilactobacillus brevis DSS.01 is a promising lactic acid bacterium for BC production with potential food-grade applications.
- This approach supports circular economy frameworks by utilizing waste streams for valuable biopolymer synthesis.
Keywords:
Box–Behnken designLevilactobacillus brevisbacterial cellulosecircular economyoptimisationresponse surface methodologyMore Related Videos
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