Related Experiment Video
Updated: Jan 29, 2026

Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
Published on: December 22, 2023
Effects of Different Crop Rotations on Microbial Diversity and Enzyme Activities in Brassica napus Rhizosphere Soil
Xiaona Tian1, Jia Duan1, Hongli Huo1
1Plant Protection Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China.
Abstract:
Continuous cropping of Brassica napus impairs sustainable production via soil nutrient imbalance and microecological degradation. We evaluated rhizosphere soil properties and microbial communities under rotations crops (Triticum aestivum [TaBn], Beta vulgaris [BvBn], Glycine max [GmBn], Sorghum bicolor [SbBn], Hordeum vulgare [HvBn], and Brassica napus [BnBn]). BvBn had the highest total nitrogen, total potassium, available potassium, and organic matter contents. TaBn exhibited the highest soil enzyme activities, and its bacterial/fungal Chao1/Simpson indices and unique operational taxonomic units (OTUs; bacteria: 333, fungi: 37) exceeded other patterns. Principal coordinate analysis showed distinct microbial community separation in BvBn/TaBn versus BnBn. TaBn enriched dominant bacterial phyla Pseudomonadota and Actinomycetota; all preceding crops increased fungal phylum Ascomycota while reducing Mucoromycota. Comprehensive assessment confirmed all preceding crops, except oilseed rape altered rhizosphere microbial structure, with TaBn as the optimal preceding crops.
More Related Videos
10:31Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
12:36High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
Published on: February 9, 2019
Related Concept Videos
The Soil Ecosystem
Enzymes and Activation Energy
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme Kinetics
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Diversity of Archaea I
Enzyme-linked Receptors
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...